Plasmid

Part:BBa_K3505043:Design

Designed by: Venetios Michelioudakis   Group: iGEM20_Thessaly   (2020-10-23)


pUPD2 BACKBONE for Golden Braid Cloning


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 2061
    Illegal SpeI site found at 14
    Illegal PstI site found at 28
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2061
    Illegal SpeI site found at 14
    Illegal PstI site found at 28
    Illegal NotI site found at 21
    Illegal NotI site found at 2067
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2061
    Illegal XhoI site found at 1045
    Illegal XhoI site found at 1937
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 2061
    Illegal suffix found in sequence at 14
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 2061
    Illegal XbaI site found at 2076
    Illegal SpeI site found at 14
    Illegal PstI site found at 28
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The Judging form didn't let us to subbmit the whole plasmids for the Best Part Collection, because the whole plasmid had inside the restriction sites that made it not combatilbe for some Assembly. So we had to subbmit separatelly the backbone and the insert.